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ADIPO-RT-PCR real time

Quantification assay for adipogenic differentiation of MSCs         cod. BM-016

Principle of the test: Quantitative analysis of FABP4A mRNA expression

Technology: Relative Quantitative PCR

Gene Target: FABP4A

Specimen: cDNA

Results: ΔΔCt method

Reporting Units: arbitrary units (AU)

Number of tests: 25 tests BM-016 

Kit storage: -20°C

Necessary equipment: 7500 Real Time PCR System

Status: Ready to use

ADIPO-RT-PCR real time  cod. BM-016

Quantification assay for adipogenic differentiation of MSCs

·      ADIPO-RT-PCR real time Quantification complete kit 25 tests BM-016

The precise predictions of the differentiation direction and potential of MSCs are an important key to the success of regenerative medicine. MSCs can differentiate into various cell types, including osteoblasts, chondrocytes, or adipocytes; therefore, they are promising as regenerative medicine. We have developed a gene-specific differentiation assay to analyze when a mesenchymal cell has switched its fate to an adipogenic lineage. We have established a novel quantitative analysis of FABP4A mRNA expression. FABP4 was initially found in murine 3T3-L1 adipocytes37 and was later discovered to be expressed in human subcutaneous adipose tissue. It is a cytosolic protein, which acts as a chaperone to guide fatty acid uptake by cells and is involved in the process of lipolysis. This method is based on real-time PCR. The expression levels of the mRNAs were determined from the threshold cycle (Ct), and the relative expression levels were calculated using the 2-ΔΔCt method. For mRNA quantification, the Ct values were normalized to the expression of the GAPDH mRNA level. Results are expressed in corresponding arbitrary units (AU) User friendly and complete, the ADIPO-RT-PCR real time Quantification kit is suitable for any laboratory.

Reference

1.      Matarese, V., Bernlohr, D. a Purification of murine adipocyte lipid-binding protein. Characterization as a fatty acid- and retinoic acid-binding protein. J Biol Chem. 263, (28), 14544-14551 (1988).

2.      Baxa, C., et al. Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry. 28, (22), 8683-8690 (1989).