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MULTISTEM cod. BM-002 - Multiplex One Step RT-PCR Real Time Cod. BM-002
Principle of the test : Quantitative analysis of stemness genes
Technology : Multiplex One Step RT- PCR Real Time
Gene Target : NANOG OCT4 SOX2
Internal Control: GAPDH
Specimen : RNA
Results : ΔΔCt method
Reporting Units : Arbitrary Units (AU)
Number of tests : 25 tests BM-002
Kit storage : -20°C
Necessary equipment : 7500 Real Time PCR System or CFX96 DetectionSystem
MULTISTEM Multiplex One Step RT-PCR Real Time cod. BioMole-002
Quantification assay of stemness markers NANOG OCT4 SOX2
In human and mouse embryonic stem cells (ESCs), Oct4, Sox2 and Nanog form a core transcriptional regulatory circuitry in pluripotent stem cells to maintain their self-renewal. Oct4 and Sox2 co-occupy a large number of enhancers/promoters and regulate the expression levels of their target genes.
These transcription factors are highly expressed in embryonic stem cells; in contrast, they are mainly silenced in normal somatic cells, except in small groups of adult stem cell populations.
We have established a novel quantitative analysis of NANOG, OCT4 and SOX2 mRNA expression. This method is based on real-time PCR. The expression levels of the mRNAs were determined from the threshold cycle (Ct), and the relative expression levels were calculated using the 2-ΔΔCt method. For mRNA quantification, the Ct values were normalized to the expression of the GAPDH mRNA level. Results are expressed in corresponding arbitrary units (AU) User friendly and complete, the MULTISTEM Multiplex One step RT-PCR real time kit is suitable for any laboratory.
Reference
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