IFNsig. type II One Step Multiplex RT-PCR Real Time
Quantitative analysis of Interferon Stimulated Genes ISG Cod. BM-024
Principle of the test : Quantitative analysis of Interferon-γ Stimulated Genes ISG
Technology : Multiplex One Step RT- PCR Real Time
Gene Target : IFNγ, IDO1, CXCL9, CXCL10, STAT1, HLA DRA
Internal Control: GAPDH
Specimen : RNA
Results : ΔΔCt method
Reporting Units : Arbitrary Units (AU)
Number of tests : 25 tests BM-024
Kit storage : -20°C
Necessary equipment : CFX96 Detection System, 7500 Real Time PCR System or all major Real-Time PCR platforms
Contacts:
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e-Mail: info@biomole.it
biomole@ALL RIGHT RESERVED 2019
IFNsig. Type II One Step Multiplex RT-PCR Real Time cod. BM-024
Quantitative analysis of Interferon-γ Stimulated Genes ISG
Type II interferon (IFN), also known as IFN-γ, is a key cytokine in the innate immune response. It is primarily produced by natural killer (NK) cells during infections and by activated T cells, NK cells, and NK T cells within the tumour microenvironment. It plays a crucial role in immune coordination and also stimulates the expression of immunosuppressive molecules such as IDO1, CXCL9, and CXCL10—collectively forming the IFN-γ signature (or type II IFN signature). A comprehensive transcriptome profile of B cells from patients with primary Sjögren's syndrome (pSS) was recently presented by Imgenberg-Kreuz et al., revealing significant differences in gene expression compared to controls, including alterations in type I and type II IFN signature targets.
The IFNsig.Type II Multiplex One Step kit is a research use only (RUO) real-time PCR assay able to reverse-transcribe and amplify RNA in a single step. Thanks to the multiplex version, the RNA is tested with two different mixes that will return the data of the six genes stimulated by Interferon α and the housekeeping gene. The relative quantification of the gene expression of these targets is performed with the ΔΔCt method and the fold change obtained is expressed in arbitrary units.
Reference:
1. Lee AJ, Ashkar AA. The Dual Nature of Type I and Type II Interferons. Front Immunol. 2018 Sep 11;9:2061. doi: 10.3389/fimmu.2018.02061
2. Imgenberg-Kreuz J, Sandling JK, Björk A, Nordlund J, Kvarnström M, Eloranta ML, Rönnblom L, Wahren-Herlenius M, Syvänen AC, Nordmark G. Transcription profiling of peripheral B cells in antibody-positive primary Sjögren's syndrome reveals upregulated expression of CX3CR1 and a type I and type II interferon signature. Scand J Immunol. 2018 May;87(5):e12662. doi: 10.1111/sji.12662.
3. Min Ae Lee-Kirsch. The Type I Interferonopathies. Annual Review of Medicine 2017;68:297-315
4. PIn A, Monasta L, Taddio A, Piscianz E, Tommasini A, Tesser A. An Easy and Reliable Strategy for Making Type I Interferon Signature Analysis Comparable among Research Centers. Diagnostics (Basel). 2019;9(3):113. doi: 10.3390/diagnostics9030113.
5. Kenneth J. Livak and Thomas D. Schmittgen. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-[Delta][Delta]CT Method. Methods, 25(4):402 – 408, 2001