Contatti:

cell: +39 329 0364389​

info@biomole.it

logo
logo

biomole@ALL RIGHT RESERVED 2019

cookie Policy | Privacy policy

201904151249411

IFNsig. type I One Step cod. BM-023 - Multiplex One Step RT-PCR Real Time            Cod. BM-023

 

Principle of the test : Quantitative analysis of Interferon Stimulated Genes ISG

Technology : Multiplex One Step RT- PCR Real Time

Gene Target : IFIT1 IFI44L IFI27 ISG15 RSAD2 SIGLEC

Internal Control: GAPDH RPS18

Specimen : RNA

Results : ΔΔCt method  

Reporting Units : Arbitrary Units (AU)

Number of tests : 25 tests BM-023

Kit storage : -20°C

Necessary equipment : 7500 Real Time PCR System or CFX96 DetectionSystem

 

IFNsig. Type I One Step cod. BM-023
Multiplex One Step RT-PCR Real Time Six Targets Two tubes

 

Type I interferonopathies are characterized by an increase of circulating type I interferon (IFN) concentration. Type I interferonopathies refer to rare Mendelian genetic disorders such as Aicardi-Goutières Syndrome (AGS) as well as more frequent and polygenic auto-immune diseases like systemic lupus erythematosus (SLE). Detection of type I IFN in these patients remains challenging as its amount is usually very low in patients' sera. Thus, the detection of interferon-stimulating genes has been proposed as an alternative for the detection of this cytokine but sensitivy, specificity and predictive values of the assay have not been reported so far (1).

 

The IFNsig.Type I Multiplex One Step kit is a real-time PCR assay able to reverse-transcribe and amplify RNA in a single step. Thanks to the multiplex version, the RNA is tested with two different mixes that will return the data of the six genes stimulated by Interferon α and the housekeeping gene. The relative quantification of the gene expression of these targets is performed with the ΔΔCt method (5) and the fold change obtained is expressed in arbitrary units. Easy to use and complete, the IFNsig.Type I One Step kit is an optimal solution for all molecular diagnostics laboratories.

 

Reference:

1.       Pescarmona R, Belot A, Villard M, Besson L, Lopez J, Mosnier I, Mathieu AL, Lombard C, Garnier L, Frachette C, Walzer T, Viel S. Comparison of RT-qPCR and Nanostring in the measurement of blood interferon response for the diagnosis of type I interferonopathies. Cytokine. 2018 Nov 6. pii: S1043-4666(18)30412-5. doi: 10.1016/j.CYto.2018.10.023

 

2.       Tovo PA, Garazzino S, Daprà V, Pruccoli G, Calvi C, Mignone F, Alliaudi C, Denina M, Scolfaro C, Zoppo M, Licciardi F, Ramenghi U, Galliano I, Bergallo M. COVID-19 in Children: Expressions of Type I/II/III Interferons, TRIM28, SETDB1, and Endogenous Retroviruses in Mild and Severe Cases. Int J Mol Sci. 2021 Jul 13;22(14):7481. doi: 10.3390/ijms22147481.

 

3.       Min Ae Lee-Kirsch. The Type I Interferonopathies. Annual Review of Medicine 2017;68:297-315

 

4.       PIn A, Monasta L, Taddio A, Piscianz E, Tommasini A, Tesser A. An Easy and Reliable Strategy for Making Type I Interferon Signature Analysis Comparable among Research Centers. Diagnostics (Basel). 2019;9(3):113. doi: 10.3390/diagnostics9030113.

 

5.       Kenneth J. Livak and Thomas D. Schmittgen. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-[Delta][Delta]CT Method. Methods, 25(4):402 – 408, 2001

BM-023 Customer presentation