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MULTISTEM cod. BM-002 - Multiplex One Step RT-PCR Real Time            Cod. BM-002


Principle of the test : Quantitative analysis of stemness genes

Technology : Multiplex One Step RT- PCR Real Time

Gene Target : NANOG OCT4 SOX2

Internal Control: GAPDH

Specimen : RNA

Results : ΔΔCt method  

Reporting Units : Arbitrary Units (AU)

Number of tests : 25 tests BM-002

Kit storage : -20°C

Necessary equipment : 7500 Real Time PCR System or CFX96 DetectionSystem

MULTISTEM Multiplex One Step RT-PCR Real Time cod. BioMole-002 


Quantification assay of stemness markers NANOG OCT4 SOX2


In human and mouse embryonic stem cells (ESCs), Oct4, Sox2 and Nanog form a core transcriptional regulatory circuitry in pluripotent stem cells to maintain their self-renewal. Oct4 and Sox2 co-occupy a large number of enhancers/promoters and regulate the expression levels of their target genes.

These transcription factors are highly expressed in embryonic stem cells; in contrast, they are mainly silenced in normal somatic cells, except in small groups of adult stem cell populations.

We have established a novel quantitative analysis of NANOG, OCT4 and SOX2 mRNA expression. This method is based on real-time PCR. The expression levels of the mRNAs were determined from the threshold cycle (Ct), and the relative expression levels were calculated using the 2-ΔΔCt method. For mRNA quantification, the Ct values were normalized to the expression of the GAPDH mRNA level. Results are expressed in corresponding arbitrary units (AU) User friendly and complete, the MULTISTEM Multiplex One step RT-PCR real time kit is suitable for any laboratory.





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